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Recombinant Protein Upscale Center (RPUC)

Recharge center assists UW researchers and beyond with recombinant protein production and purification needs. Beneficial to researchers who have E. coli expression constructs known to express soluble protein.

Visit Facility

Description

RPUC will prepare large scale growth (2 liters ea) of E. coli constructs with independent an air source, pellet cultures, wash, and screen for recombinant protein, (His-tagged) on SDS page gel. RPUC can also perform a simple IMAC or a more extensive SEC purification on the soluble fraction.

Key Words

Service benefits those with prepared/screened recombinant E. coli expression strains with known protein expression levels

Details

Type
  • Services
Administrator
Campus Seattle
College / School / Unit School of Medicine
Location
  • Building E, E610
Availability
  • UW and external users
Category
  • Protein and chemical analysis
Year Started 2006
Director / PI

Contact Us

For webpage updates please email researchcommunity@uw.edu