Description

The CRISPR, Vector and Transgenic Mouse Core provides the following to Diabetes Research Center affiliate investigators:

• Production of CRISPR/Cas9 reagents for genome editing in cells and preclinical models;
• Production of CRISPR reagents for various other applications, such as CRISPR-mediated transcriptional activation and repression;
• Production of adeno-associated viral (AAV) and lentiviral vectors for use in preclinical models, tissues and cells;
• CRISPR/Cas9-directed gene editing in mice through a collaboration with the UW Mouse CRISPR Core;
• Generation of cell lines by CRISPR/Cas9 in collaboration with the Institute for Stem Cell & Regenerative Medicine (ISCRM);
• Development of genotyping methods, genotyping and sequencing to validate edited models;
• Other molecular biology techniques not routinely performed in affiliate investigators’ laboratories;
• Consultation at each step;
• Training of affiliates and their trainees in the methods used by the Core. Investigators who are not DRC affiliates may also use the Core’s services as time permits.

Key Words

CRISPR/Cas9 services, Gene editing, Adeno-associated virus production, Lentivirus production, Genotyping