Description

The MANTIS Lab houses the Spatial Biology Core and the Pathology Research Services Laboratory. The Spatial Biology Core provides tissue interrogation services for spatial profiling experiments of RNA and protein in tissue sections using the Bruker GeoMx DSP and CosMx SMI platforms. The facility is located in the E-building of the University of Washington’s South Lake Union (SLU) campus and is staffed by experienced research scientists. The core facility is overseen by pathologists with hands-on experience executing spatial profiling experiments using spatial profiling on human and mouse tissues. The core facility is located adjacent to the Pathology Research Services laboratory enabling seamless data generation from submitted tissues/tissue blocks as well as downstream validation of spatial profiling experiments’ results using in situ hybridization (ISH) and immunohistochemistry (IHC).

The primary morphologic techniques offered by the Pathology Research Services Laboratory, include paraffin and frozen tissue processing and embedding, routine histology including special histologic stains, tissue enzyme histochemistry, immunohistochemistry (IHC), in situ hybridization (ISH), immunofluorescence (IF), TUNEL staining, and ancillary techniques (such as competitive inhibition assays and Western blotting necessary to ensure the specificity and sensitivity of reagents). Scientists and staff in our lab have many years of experience and expertise in all of these.

Key Words

GeoMx, CosMx, Spatial Transcriptomics, Spatial Proteomics, Immunohistochemistry, Immunofluorescence, In Situ Hybridization, Precision Slice Culture, Slide Scanning, Consultation, Pathology Services