Description

Single-molecule, real-time (SMRT) sequencing is a third-generation sequencing technology that generates long-read (ten thousand to a hundred thousand bases) DNA and cDNA sequences. With the PacBio Revio instrument, we can generate high-quality single-molecule consensus sequences (HiFi) of genomic DNA (~18 kbp) for de novo whole-genome assembly, full-length cDNA (up to 10 kbp long) for isoform discovery and genome annotation, and amplicons up to 10 kbp long. Oxford Nanopore Technologies (ONT) provides third-generation sequencing reads ranging from tens to hundreds of thousands of base pairs long. Both platforms also provide single-molecule epigenetic information (i.e., CpG methylation calls). For most protocols, DNA samples are not PCR amplified, leading to reduced GC bias and improved sensitivity in complex or repetitive DNA regions. We offer QC and library preparation from DNA or RNA in addition to sample sequencing.

We provide library preparations and sequencing for:

• High-Fidelity (HiFi) Template Preparation (15-22 kbp)
• Transcriptome and isoform sequencing with PacBio’s full-length RNA Kinnex protocol
• Purified PCR products
• Large-insert gDNA (20 kbp+)
• Ampli-Fi ultra-low-input protocol
• Large projects with a high sample number
• Multiplex amplicons or small genomes; multiplex up to 384 samples

Key Words

PacBio Revio, DNA Sequencing, cDNA Sequencing, Kinnex, Long Read Sequencing, Sample QC, DNA, RNA, Whole Genome, HiFi, FEMTO Pulse, ONT